Comparative Study of Nicotine Content in Moldavian Tobacco by UVVis Spectrophotometry and UHPLC and their Mineral Composition by ICP-OES

Ciocarlan, A.; Shvetsova, M.; Aricu, A.; Lungu, L.; Peshkova, A.; Zinicovscaia, I.; Deleanu, M.; Duca, G.

Авторы: Ciocarlan Alexandru, Shvetsova Margarita, Aricu Aculina, Lungu Lidia, Peshkova Alexandra, Zinicovscaia Inga, Deleanu Mariana, Duca Gheorghe
Журнал: Biological Trace Element Research
Год издания: 2025
Издательство: Humana Press, Inc.
Местоположение издательства: United States
DOI: 10.1007/s12011-025-04880-y
Аннотация: Tobacco is one of the oldest cultures known to mankind for more than 5000 years. Despite the proven harm by smoking,the number of smokers in Moldova is growing, making the quality control of tobacco products an important task. Thisstudy focused on the quantification of nicotine and the determination of the mineral composition in autochthonous Moldavian tobacco selections. Ultraviolet–visible (UV-Vis) spectroscopy and ultrahigh performance liquid chromatography(UHPLC) were applied for quantification of nicotine in ten tobacco varieties (Molovata, Trapezond 209, Doina 210, Moldavschi 237, Virginia 263, Moldavschi 272, Burley 320, Virginia 401, Moldavschi 456, Jubileu M) and Nicotiana rusticaL. species of Moldavian origin. The nicotine content determined by UV-Vis analysis ranged from 1.24% to 2.74%, whileUHPLC analysis yielded a range of 0.97% to 2.16% and the difference is due to the higher selectivity and accuracy of theUHPLC method. A total of 14 chemical elements, Al, S, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Sr, Cd, Ba and Hg, were identifiedin tobacco varieties by inductively coupled plasma–optical emission spectrometry. The mean elements content in tobaccoleaves samples was the following: Al (1390±795 mg/kg), S (3043±749 mg/kg), V (1.9±1.1 mg/kg), Cr (2.5±1.3 mg/kg), Mn (126±21 mg/kg), Fe (1077±579 mg/kg), Co (0.39±0.19 mg/kg), Ni (2.4±0.9 mg/kg), Cu (3.9±1.5 mg/kg), Zn(13.8±3.3 mg/kg), Sr (164±17 mg/kg), Cd (1.4±0.3 mg/kg), Ba (62±12 mg/kg) and Hg (0.04±0.005 mg/kg). Principalcomponents analysis, applied for element grouping, revealed two groups of elements, which can be associated with elements uptake by plants from soil, agricultural practices, fuel combustion, and vehicles and road dust.
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Comparative Study of Nicotine Content in Moldavian Tobacco by UVVis Spectrophotometry and UHPLC and their Mineral Composition by ICP-OESстатья

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