Аннотация:The global carbon cycle is aided by soil autotrophic bacteria, which are crucial for storing atmospheric CO 2. There are few reports of carbon-fixing bacteria in agro-soils, despite the fact that their potential to improve soil fertility has not been thoroughly investigated. The response of carbon-fixing bacterial populations to nitrogen addition in agricultural croplands is unknown. In order to investigate the effects of organic (cellulose) and ammonium salt solution treatments on soil organic carbon (SOC) and autotrophic soil bacterium abundance, a 54-day laboratory microcosm experiment was created. The quantity of crucial carbon cycle genes, such as cbbL and cbbM, is influenced by ammonium and organic inputs, which in turn alters the microbial community. The main soil factors affecting the quantity of carbon-fixing bacteria were the SOC and microbial biomass carbon. These results suggest that adding cellulose, the primary ingredient in wheat straw, and fertilizing with nitrogen may raise SOC, enhancing the soil fertility and carbon content of agricultural soil. According to the findings, more investigation is required to ascertain how ammonium and plant residues impact the diversity and activity of the soil's autotrophic microbial communities as well as how to manage them. The information can be used to determine safe and efficient dosages of ammonium fertilizers and plant wastes for modern, environmentally friendly agriculture in central Russia.