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Effects of fibrinogen, thrombin and ε-aminocaproic acid on the viability of human keratino- cytes, fibroblasts and endothelial cellsстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: EV Solovieva, Rudyak SG, Pavlov N., Phedotov A., Paltsev MA, Komlev V., Panteleyev AA
- Журнал: Journal of Investigative Dermatology
- Том: 135
- Год издания: 2015
- Издательство: Blackwell Publishing Inc.
- Местоположение издательства: United Kingdom
- Первая страница: S30
- Последняя страница: S30
- Аннотация: One of the most desired features of polymeric scaffolds used in skin bioengineering is their ability to support the ingrowth of blood vessels and thus to ensure the effective engraftment of skin equivalent after transplantation. We designed a number of sponge scaffolds using sodium alginate as a basic material with addition of fibrinogen, thrombin and ε-aminocaproic acid as pro-angiogenic microenvironmental components. The scaffolds were crosslinking using glutaraldehyde, Ca2+ and Mg2+ ions. The porosity of obtained matrixes was 60-70% with pore size between 30-400 μm. The features of the matrices such as degradation rates, changes of mechanical properties and ability to support growth of human keratinocytes (N-TERT), primary fibroblasts and cells spreading from mouse aorta rings (mostly endotheliocytes) in long-term 3D culture have been compared. Cellviability was assessed using fluorescein diacetate labeling. Our results showed that all alginate-fibrinogen matrixes (containing from 15 to 85% of alginate and correspondingly from 85 to 15% of fibrinogen) were effectively colonized by fibroblasts and keratinocytes if their pore size stays between 30 and 150 μm. The introduction of ε-aminocaproic acid actively supports spreading of the mouse aorta ring cells over the matrix volume but blocks the keratinocyte ability to adhere to and fibroblasts ability to migrate into the alginate/fibrinogen scaffolds. The addition of thrombin leads to significant reduction of average pore diameter in alginate/fibrinogen matrixes (less than 50 μm) and supports keratinocyte adhesion and fibroblast migration along with promotion of spreading of mouse aorta ring cells. Thus, in combination with pro-angiogenic components (e.g., fibrinogen and thrombin)alginate-based sponge matrices may represent an efficient (and also cost-effective) alternative to collagen-based scaffolds for skin bioengineering.
- Добавил в систему: Соловьева Елена Викторовна