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Autologous platelet-rich plasma as an effective pro-angiogenic and wound healing component of composite alginate sponge scaffoldsстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: EV Solovieva, Teterina A., Klein O., Komlev V., Panteleyev AA
- Журнал: Journal of Investigative Dermatology
- Том: 138
- Год издания: 2018
- Издательство: Blackwell Publishing Inc.
- Местоположение издательства: United Kingdom
- Аннотация: Platelet rich plasma (PRP) obtained from patient’s own blood has prominent, yet poorlyunderstood positive effects upon chronic wound healing in diabetic patients. These effectscan be associated with multiple growth factors (PDGF, EGF, FGF, VEGF, TGF-b, etc.) enrichedin the PRP. Supplementation with recombinant growth factors is also a useful approach inbioengineering of dermal substitutes used for wound healing therapy, but it is costly and not safe. Here, we have assessed the pro-angiogenic and wound healing properties of sodium alginate polymeric sponge scaffolds modified with human PRP in comparison with appropriate controls. The scaffolds were obtained by freeze-drying and crosslinking using 2+ cations. Scaffold porosity was about 90-95% with pore size between 80-350 mm. PRP promoted proliferation of human primary fibroblasts, subcutaneous mesenchymal stromal cells and epidermal keratinocytes, as well as adhesion of these cells to the scaffolds. PRP supplementation showed ability to support vascularization of the scaffolds implanted under themouse skin in 10 days-long tests. For estimation of wound healing properties, the 5-mm diameter sponge scaffolds were placed on full-thickness skin wounds of the same size on the back of 8-12 week-old SCID mice. Wound closure rates, formation of granulation tissue and scaffold neovascularization were assessed on days 3, 6, and 9. PRP supplementation hassignificantly prompted the rates of wound closure and scaffold neovascularization, along with increased volume of granulation of tissue. Thus, the use of autologous human PRP obtained from the patient may represent an efficient, safe and cost-effective alternative to scaffold supplementation with recombinant growth factors in bioengineered dermal equivalents for wound healing therapy.
- Добавил в систему: Соловьева Елена Викторовна