Аннотация:The response of biological activity and the variability of the content of labile forms of biogenic elements in peats of different genesis to variations in their moisture content have been studied under laboratory conditions. Samples of peat soils of the northern and southern taiga (Tyumen and Moscow oblasts) have been analyzed. A series of manipulation experiments have been conducted to estimate dynamics of basal respiration (BR), microbial biomass carbon, and labile carbon and nitrogen content in different peat types at different moisture levels. The effects of the experiment duration and type on the dynamics of peat properties have also been assessed. In all studied peat samples, the maximum levels of biological activity have been recorded in a wide range of moisture content. In general, they fall within the range from 20–30 to 80–90% of water–holding capacity (WHC). In this range, no significant relationship between moisture content and BR has been detected. In most experiments, a reliable reduction of biological activity was observed only in the “extreme” humidity ranges—less than 20% or more than 90% WHC. Furthermore the response to moistening is individual, determined by the sample properties, and is little affected by the type of sample processing. In experiments and model calculations, it is proposed to use the obtained sensitivity coefficient to moisture (W20), which in the range of 40‒80% WHC is 1.0‒1.2 for peat samples of different genesis. Long-term incubation experiments revealed a more pronounced response to over-moistening: the reduction of biological activity at high humidity values (more than 80% WHC) was more reliable. This may indicate their better applicability when addressing problems of modeling the behavior of ecosystems with a change in the hydrological situation.