Genomic Composition of the Artificial Hybrid ×Trititrigiacziczinii (Hordeeae, Poaceae) and Related Taxa According to Molecular Phylogenetic Data

Gnutikov, A.A.; Nosov, N.N.; Zuev, E.V.; Lysenko, N.S.; Shneyer, V.S.; Troitsky, A.V.; Rodionov, A.V.

Авторы: Gnutikov Alexander A., Nosov Nikolai N., Zuev Evgeny V., Lysenko Natalia S., Shneyer Victoria S., Troitsky Aleksey V., Rodionov Alexander V.
Журнал: Plants
Том: 15
Номер: 1
Год издания: 2026
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Номер статьи: 70
DOI: 10.3390/plants15010070
Аннотация: ×Trititrigia cziczinii Tzvelev is a promising crop developed through distant hybridizationbetween Elytrigia intermedia (Host) Nevski (=Thinopyrum intermedium (Host) Barkworth &D.R. Dewey) and Triticum aestivum L., followed by backcrossing with wheat. This studyelucidates the genomic composition of this hybrid and its parental taxa using molecularphylogenetic analysis of nuclear (ITS, ETS) and chloroplast (trnK–rps16, ndhF) DNA markers,complemented by Next-Generation Sequencing (NGS) of the 18S–ITS1–5.8S rDNAregion. Results from Sanger sequencing revealed that the primary nuclear ribosomal DNA(rDNA) of the hybrid originates from Triticum aestivum; a finding strongly supportedby both Bayesian inference and Maximum Likelihood analyses. Chloroplast DNA dataunequivocally indicate maternal inheritance from T. aestivum. In contrast, ETS sequenceanalysis showed phylogenetic affinity to Elytrigia intermedia, suggesting complex genomicreorganization or chimeric sequence formation in the hybrid. NGS data corroborate thedominance of T. aestivum-like ribotypes in the hybrid’s rDNA pool, with only a minorfraction identical to the main ribotype of E. intermedia. Genetic structure analysis furtherrevealed geographic heterogeneity in the genomic composition of E. intermedia populations.The predominance of the wheat genome in ×T. cziczinii is likely a consequenceof stabilizing backcrosses and illustrates a case of rDNA elimination from one parentalgenome during hybridization. This research underscores the complex genomic dynamicsin artificial hybrids and the utility of multi-marker phylogenetic approaches for clarifyingtheir origins.
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Genomic Composition of the Artificial Hybrid ×Trititrigiacziczinii (Hordeeae, Poaceae) and Related Taxa According to Molecular Phylogenetic Dataстатья Исследовательская статья

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