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A highly expressed antennae chemosensory protein 6 of Dastarcus helophoroides Is involved in the recognition of herbivore-induced plant volatilesстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 23 января 2026 г.
- Авторы: Wu Y.X., Liu Q., Wickham J.D., Li J Y., Kuerban K., Xu Z.X., Li S, Wang L.J., Lv N.N., Fan J.T.
- Журнал: Journal of Agricultural and Food Chemistry
- Том: 73
- Номер: 39
- Год издания: 2025
- Издательство: American Chemical Society
- Местоположение издательства: United States
- Первая страница: 24572
- Последняя страница: 24584
- DOI: 10.1021/acs.jafc.5c07176
- Аннотация: Dastarcus helophoroides, an ecologically friendly biocontrol vector, precisely locates hosts via olfaction-mediated chemoreception to complete its life cycle. Chemosensory proteins (CSPs) mediate this process by binding and transporting odorants. This study characterizes DhelCSP6’s role in perceiving herbivore-induced plant volatiles (HIPVs) in D. helophoroides. Quantitative real-time PCR (qRT-PCR) and fluorescence competitive binding assays demonstrated that DhelCSP6, expressed specifically in antennae, binds four of the eight HIPVs. Three-dimensional (3D) structural modeling, molecular docking, and molecular dynamics (MD) simulations revealed that DhelCSP6 features six typical α-helices forming a hydrophobic ligand-binding pocket, with six key residues (Ile89, Leu67, Leu104, Met50, Tyr46, and Tyr108) stably interacting with the four ligands via van der Waals forces. Electroantennographic (EAG) and dual-choice biobehavioral assays demonstrated D. helophoroides perception of and response to p-cymene, γ-terpinene, and fenchone, while DhelCSP6 silencing abolished ligand perception. These findings establish DhelCSP6 as integral to olfactory recognition and chemoreception in D. helophoroides.
- Добавил в систему: Викам Джэйкоб Дэвид