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Isolation of Methanotrophic Consortium from Chernevaya Taiga Soil and Laboratory Research on Its Introduction into Agro-Soilстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 23 января 2026 г.
- Авторы: Kravchenko Irina K., Gogmachadze Liana G., Zverev Aleksei O., Sukhacheva Marina V., Lapidus Alla L.
- Журнал: Microorganisms
- Том: 13
- Номер: 9
- Год издания: 2025
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Первая страница: 2052
- Последняя страница: 2052
- Номер статьи: 2052
- DOI: 10.3390/microorganisms13092052
- Аннотация: Aerobic soils serve as significant sinks for atmospheric methane, with their effectiveness influenced by the diversity and activity of soil methanotrophs. Land-use changes, particularly the conversion of natural ecosystems to agriculture, can substantially alter these microbial communities. A promising strategy to restore methane oxidation capacity is the introduction of active, ambient methane-oxidizing bacteria. The stable methane-oxidizing microbial consortium T1, dominated by Methylocystis (74%), was isolated from the soil of the unique Chernevaya Taiga forest ecosystem. The effects of inoculating this consortium were evaluated in a four week laboratory incubation experiment, using microcosms of soddy-podzolic agro-soil. Methane oxidation potential was assessed to measure methanotroph activity; methanotrophs were quantified using qPCR targeting pmoA genes; and the diversity of soil microbial communities was examined through 16S rRNA gene profiling. Inoculated soils exhibited significantly higher methane oxidation potentials compared to non-inoculated soils. Furthermore, pmoA gene copy numbers in the inoculated soils were significantly elevated (106 copies pmoA g−1), indicating stable persisted methanotrophic populations throughout the incubation period. These findings suggest that enriched methanotrophic consortium inoculation into agro-soils may be a promising strategy for restoring methane-oxidizing activity.
- Добавил в систему: Гогмачадзе Лиана Гуладиевна