Properties of Heterochannels Kv(1.1-1.2)2 with Mutation T226R in the Kv1.1 Subunit

Ignatova, A.A.; Efremenko, A.V.; Abramochkin, D.V.; Dzhumaniiazova, I.; Shmatin, I.I.; Kirpichnikov, M.P.; Feofanov, A.V.; Nekrasova, O.V.

Авторы: Ignatova Anastasia A., Efremenko Anastasia V., Abramochkin Denis V., Dzhumaniiazova Irina, Shmatin Ivan I., Kirpichnikov Mikhail P., Feofanov Alexey V., Nekrasova Oksana V.
Журнал: International Journal of Molecular Sciences
Том: 26
Номер: 19
Год издания: 2025
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 9730
Последняя страница: 9730
DOI: 10.3390/ijms26199730
Аннотация: AbstractMutation T226R in the Kv1.1 α-subunit of voltage-gated potassium Kv1 channels is associated with episodic ataxia type 1, severe neuromyotonia, and epilepsy. In vitro, this mutation was reported to considerably distort the functioning of homotetrameric channels Kv1.1; however, in the brain, Kv1.1 α-subunits form heterochannels predominantly associating with Kv1.2 α-subunits. Using the patch-clamp technique, fluorescent and Förster resonance energy transfer confocal microscopy, we revealed that heterochannels Kv(1.1(T226R)-1.2)2 formed by concatemers Kv1.1(T226R)-Kv1.2 in Neuro-2a cells have significantly slower activation and deactivation rates, and their activation occurs at a much less negative membrane potential compared to channels Kv(1.1-1.2)2 formed by concatemers Kv1.1-Kv1.2. This mutation does not noticeably affect the formation of complexes between α-subunits Kv1.1 and Kv1.2, but it does induce a delayed and possibly decreased presentation of heterochannels Kv(1.1(T226R)-1.2)2 on the plasma membrane. At the same time, the T226R mutation has a much stronger negative effect on the membrane presentation of homotetrameric Kv1.1 channels. Since heterochannels Kv1.1-Kv1.2 but not homotetrameric channels Kv1.1 are present in the brain, the heterochannels bearing mutation T226R are most likely underlying the pathogenesis of the disease by decreasing the responsiveness of cells to mild membrane depolarization and, thus, increasing the excitability of neurons.
Добавил в систему: Джуманиязова Ирина Хамрабековна

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Properties of Heterochannels Kv(1.1-1.2)2 with Mutation T226R in the Kv1.1 Subunitстатья

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