Biotechnological Approaches to Plant Antiviral Resistance: CRISPR-Cas or RNA Interference?

Kalinina, N.O.; Spechenkova, N.A.; Taliansky, M.E.

Авторы: Kalinina Natalia O., Spechenkova Nadezhda A., Taliansky Michael E.
Журнал: Biochemistry (Moscow)
Том: 90
Номер: 6
Год издания: 2025
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 804
Последняя страница: 817
Оригинальные версии: БИОТЕХНОЛОГИИ УСТОЙЧИВОСТИ РАСТЕНИЙ К ВИРУСАМ: CRISPR-Cas ИЛИ РНК-ИНТЕРФЕРЕНЦИЯ
DOI: 10.1134/s0006297925600139
Аннотация: Established genome editing technologies, such as CRISPR-Cas and RNA interference (RNAi), have significantly advanced research studies in nearly all fields of life sciences, including biotechnology and medi- cine, and have become increasingly in demand in plant biology. In the review, we present the main principles of the CRISPR-Cas and RNAi technologies and their application in model plants and crops for the control of viral diseases. The review explores the antiviral effects they provide, including direct suppression of genomes of DNA- and RNA-containing viruses and inhibition of activity of host genes that increase plant susceptibil- ity to viruses. We also provide a detailed comparison of the effectiveness of CRISPR-Cas and RNAi methods in plant protection, as well as discuss their advantages and disadvantages, factors limiting their application in practice, and possible approaches to overcome such limitations.
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Biotechnological Approaches to Plant Antiviral Resistance: CRISPR-Cas or RNA Interference?статья

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