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Kinetics of merthiolate-induced aggregation of human plateletsстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 5 декабря 2014 г.
- Авторы: Vrzheshch P.V., Tatarintsev A.V., Orlova E.V., Ershov D.E., Varfolomeev S.D.
- Журнал: Thrombosis Research
- Том: 67
- Номер: 5
- Год издания: 1992
- Издательство: Pergamon Press
- Местоположение издательства: United States
- Первая страница: 505
- Последняя страница: 516
- DOI: 10.1016/0049-3848(92)90012-Y
- Аннотация: Incubation of human platelet-rich plasma (PRP) or washed platelets with merthiolate (MT; sodium ethylmercurithiosalicylate; an inhibitor of lysophosphatide: arachidonoyl transferase) leads to irreversible platelet aggregation which is parallelled by an increase in thromboxane A2 synthesis. MT-induced aggregation is preceded by a pronounced lag-period (0.5–10 min). Duration of the latter is inversely related to the concentration of MT ([MT]). Platelet responses to MT are similar to those triggered by arachidonate (AA) in that the relationships of the aggregation rates both to [MT ] and [AA] are threshold and exhibit characteristic super-high values of the apparent Hill coefficients (h > 30). A typical MT-induced response can be subdivided in two sequential phases: i) cyclooxygenase-independent slow aggregation, and ii) indomethacin-abrogated rapid aggregation. MT-induced responses are blocked by PGE1 or ajoene (which inhibits binding of fibrinogen to its cell surface receptor, GPIIb/IIIa). The obtained data are interpreted both quantitatively and qualitatively in terms of a model assuming the existence of: i) a relationship between the rate of MT-inhibitable AA incorporation into phospholipids and the concentration of intracellular free AA, [AA] i ; ii) a certain threshold value of [AA] i essential for triggering the second phase of the aggregation.
- Добавил в систему: Вржещ Петр Владимирович