Combined Effects of Drying–Rewetting and Ammonium Addition on Methanotrophs in Agricultural Soil: A Microcosm Study

Kravchenko, I.K.; Zverev, A.O.; Gogmachadze, L.G.; Stepanov, A.L.

Авторы: Kravchenko Irina K., Zverev Aleksei O., Gogmachadze Liana G., Stepanov Aleksey L.
Журнал: AGRICULTURE-BASEL
Том: 14
Номер: 12
Год издания: 2024
Издательство: MDPI Publishing
Местоположение издательства: Basel, Switzerland, Switzerland
Первая страница: 2243
Последняя страница: 2245
Номер статьи: 2243
DOI: 10.3390/agriculture14122243
Аннотация: Oxidation of methane by soil microorganisms is an important mechanism controlling thecontent of this potent greenhouse gas in the atmosphere. Agricultural soils operate under stressful conditions, and ammonium (N-fertilization) and drying (global warming) may have a significant impacton methane oxidation. In order to investigate how soil methanotrophs respond to drying–rewetting(DW), ammonium addition (100 mg/g) (A), and their combined action (MS), agricultural soil microcosms were incubated over the three months and methane oxidation was measured before and afterperturbations, while community composition was monitoring using 16S rRNA gene sequencing. Asignificant decline in the methane-oxidation activity after perturbations was found, with subsequentrestoration, and the combined treatment was more effective than the sum of individual treatments,indicating a synergistic effect. After rewetting, the structure of the bacterial community returnedto pre-dry-down levels, but the application of ammonia and combined action lead to irreversiblechanges in the structure of soil methanotrophic communities. Methanotroph Methylomicrobium weresignificantly reduced under disturbances, while there was a significant increase in the representation of Methylobacter accompanied by the facultative methylotroph Methylovorus. We concludedthat methanotrophic communities in agricultural soil demonstrated flexibility, and even when theabundance of dominant populations drops, ecosystem functions can recover.
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