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Transcription factors ClrB and XlnR and their effect on the transcription of cellulase genes in the filamentous fungus Penicillium verruculosumстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 февраля 2025 г.
- Авторы: Chulkin A., Kislitsin V., Sinelnikov I., Sinitsyn A., Zorov I., Volkov P., Rozhkova A.
- Журнал: International Journal of Molecular Sciences
- Том: 25
- Год издания: 2024
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Номер статьи: 13373
- DOI: 10.3390/ijms252413373
- Аннотация: Abstract: The filamentous fungus Penicillium verruculosum (anamorph Talaromyces verruculosus) hasbeen shown to be an efficient producer of secreted cellulases, used in biorefinery processes. Understanding the mechanisms of regulation of cellulase gene expression in the fungus P. verruculosum isa current task in industrial biotechnology, since it allows for targeted changes in the compositionof the complex secreted by the fungus. Expression of cellulase genes in fungi is regulated mainlyat the level of transcription via pathway-specific transcription factors (TF), the majority of whichbelong to the Zn(II)2Cys6 family of zinc binuclear cluster proteins. Transcriptional regulation ofcellulase genes may have a species-specific pattern and involves several transcription factors. In thisstudy, we used a qPCR method and transcriptome analysis to investigate the effect of knockoutsand constitutive expression of genes encoding homologues of the regulatory factors XlnR and ClrBfrom P. verruculosum on the transcription of cbh1, egl2, and bgl1 genes, encoding three key cellulases,cellobiohydrolase, endoglucanase, and β-glucosidase, in the presence of various inducers. We haveshown that the transcription factor XlnR of the filamentous fungus P. verruculosum is strictly responsible for the transcription of the main cellulolytic genes (cbh1, egl2, and bgl1) in the presence of xyloseand xylobiose, but not in the presence of cellobiose. ClrB/Clr-2, a homologue from P. verruculosum,does not represent the main transcription factor regulating transcription of cellulolytic genes in thepresence of selected inducers, unlike in the cases of Aspergillus nidulans, Aspergillus niger, and Penicillium oxalicum; apparently, it has a different function in fungi from the genus Talaromyces. We havealso shown that constitutive expression of the transcription factor XlnR resulted in 3.5- and 2-foldincreases in the activity of xylanase and β-glucosidase in a B1-XlnR enzyme preparation, respectively.In a practical sense, the obtained result can be used for the production of enzyme preparations basedon the P. verruculosum B1-XlnR strain used for the bioconversion of renewable cellulose-containingraw materials into technical sugars.
- Добавил в систему: Синицын Аркадий Пантелеймонович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Экспертное заключение | Чулкин.jpg | 1,7 МБ | 23 мая 2025 [aps306cellulas] | |
| 2. | Полный текст | Transcription_Factors_ClrB_and_XlnR_and_Their_Effe.pdf | 2,1 МБ | 17 февраля 2025 [aps306cellulas] |