Poly(D,L-lactide-co-glycolide) Nanoparticles Modified by Layer-by-Layer Adsorption of Polyethyleneimine and Dextran Sulfate for Cyanocobalamin Embeddingстатья
Статья опубликована в журнале из списка Web of Science и/или Scopus
Аннотация:The layer-by-layer adsorption of polyethyleneimine and dextran sulfate onto the nanoparticles of biodegradable poly(D,Llactide-co-glycolide) was conducted to produce potential delivery system of cyanocobalamin. The modified poly(D,Llactide-co-glycolide) particles are found to be colloidal stable, nanosized with the value of hydrodynamic diameter of 210 nm and polydispersity index of 0.22. It was established that the content of cyanocobalamin absorbed by polyelectrolyte shell of the modified particles was 16 wt.%. It was observed that the cyanocobalamin loading slightly affects the physicochemical parameters of the particles. Moreover, the obtained nanosomal form of the cyanocobalamin does not require to be lyophilized; it could be stored as a dispersion due to extremely slow release of loaded active agent under normal ambient conditions at 25 °C. A comprehensive study of the poly(D,L-lactide-co-glycolide) nanoparticles modified by oppositelycharged polyelectrolytes loaded with the cyanocobalamin using dynamic light scattering, analytical ultracentrifugation, small-angle X-ray scattering and atomic force microscopy allows to evaluate the value of thickness of the polyelectrolyte shell onto the poly(D,L-lactide-co-glycolide) core, which is found to be about 30 nm. The developed nanocarriers based on the poly(D,L-lactide-co-glycolide) particles modified by the alternating adsorption of polyethyleneimine and dextran sulfatecan be considered as a promising candidate for simultaneous delivery of both hydrophobic agents loaded into the poly(D,Llactide- co-glycolide) core and water-soluble cyanocobalamin incorporated into the polyelectrolyte shell.
