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Microrheological responses of erythrocytes to gasotransmitters, by inhibiting guanylate cyclase, NO synthase and blocking ATP-dependent and calcium-dependent potassium channelsстатья Исследовательская статья
- Авторы: Muravyov A., Tikhomirova I., Volkova E., Priezzhev A., Lugovtsov A., Mikhailov P.
- Журнал: Series on Biomechanics
- Том: 38
- Номер: 4
- Год издания: 2024
- Издательство: Bulgarian Academy of Sciences
- Местоположение издательства: Bulgaria
- Первая страница: 15
- Последняя страница: 23
- DOI: 10.7546/sb.03.04.2024
- Аннотация: Background: The positive effect of gasotransmitters (GT), nitric oxide (NO) and hydrogen sulfide (H2S) on the microrheology of blood cells is known. However, the mechanisms of microrheological changes in red blood cells (RBCs) remain poorly understood. The purpose of this study was to investigate RBC microrheological responses to the action of GT donors, substrates of their endogenous synthesis and to analyze the intracellular signaling pathways associated with these processes. Methods: RBCs were separated from plasma, washed, resuspended in Ringer's solution, and aliquoted. Cells were incubated: with sodium nitroprusside (SNP, 100 M), with an H2S donor, sodium hydrosulfide (NaHS, 100 M), with an inhibitor of soluble guanylate cyclase (ODQ, 0.5 M), with ATP-dependent K blocker channels, glibenclamide (GcL, 100 M) and blocker of Ca2+-dependent K+ channels (KCa3.1) – clotrimazole (CLM, 50.0 M) The RBC deformability (RBCD) and their aggregation (RBCA) were recorded after incubation with the drugs. Results: After RBC incubation with SNP, an increase in EI by 9% was detected (p<0.01). Under these conditions, RBCA decreased by 32% (p < 0.01). An increase in EI and a decrease in RBCA were also observed after incubation of cells with NaHS, by 10 and 28%, respectively. When s-GC was inhibited with ODQ the microrheological effects of SNP and NaHS did not appear. However, blocking KATP channels did not eliminate the increase in EI and the decrease in aggregation under the influence of NaHS. While blocking KCa3.1 channels with CLM almost completely eliminated the effects of NaHS. Conclusion: Thus, the results of the study allow us to conclude that the effects of NO and H2S can be realized in red blood cells using the s-GC-cGMP signaling pathway and, perhaps through the activation of KCa3.1 channels.
- Добавил в систему: Луговцов Андрей Егорович