Yeast Glucan Remodeling Protein Bgl2p: Amyloid Properties and the Mode of Attachment in Cell Wall

Motorin, N.A.; Makarov, G.I.; Rekstina, V.V.; Evtushenko, E.G.; Sabirzyanov, F.A.; Ziganshin, R.H.; Shaytan, A.K.; Kalebina, T.S.

Авторы: Motorin Nikita A., Makarov Gennady I., Rekstina Valentina V., Evtushenko Evgeniy G., Sabirzyanov Fanis A., Ziganshin Rustam H., Shaytan Alexey K., Kalebina Tatyana S.
Журнал: International Journal of Molecular Sciences
Том: 25
Номер: 24
Год издания: 2024
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Номер статьи: 13703
DOI: 10.3390/ijms252413703
Аннотация: Bgl2p is a major, conservative, constitutive glucanosyltransglycosylase of the yeast cell wall (CW) with amyloid amino acid sequences, strongly non-covalently anchored in CW, but is able to leave it. In the environment, Bgl2p can form fibrils and/or participate in biofilm formation. Despite a long study, the question of how Bgl2p is anchored in CW remains unclear. Earlier, it was demonstrated that Bgl2p lost the ability to attach in CW and to fibrillate after the deletion of nine amino acids in its C-terminal region (CTR). Here, we demonstrated that a Bgl2p anchoring is weakened by substitution Glu-233/Ala in the active center. Using AlphaFold and molecular modeling approach, we demonstrated the role of CTR on Bgl2p attachment and supposed the conformational possibilities determined by the presence or absence of an intramolecular disulfide bond, forming by Cys-310, leading to accessibility of amyloid sequence and β-turns localized in CTR of Bgl2p for protein interactions. We hypothesized the mode of Bgl2p attachment in CW. Using atomic force microscopy, we investigated fibrillar structures formed by peptide V187MANAFSYWQ196 and suggested that it can serve as a factor leading to the induction of amyloid formation during interaction of Bgl2p with other proteins and is of medical interest being located close to the surface of the molecule.
Добавил в систему: Макаров Геннадий Иванович

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Yeast Glucan Remodeling Protein Bgl2p: Amyloid Properties and the Mode of Attachment in Cell Wallстатья Исследовательская статья

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