Oropharyngeal resistome remains stable during COVID-19 therapy, while fecal resistome shifts toward a less diverse resistotype

Starikova, E.V.; Galeeva, Y.S.; Fedorov, D.E.; Korneenko, E.V.; Speranskaya, A.S.; Selezneva, O.V.; Zoruk, P.Y.; Klimina, K.M.; Veselovsky, V.A.; Morozov, M.D.; Boldyreva, D.I.; Olekhnovich, E.I.; Manolov, A.I.; Pavlenko, A.V.; Kozlov, I.E.; Yanushevich, O.O.; Krikheli, N.I.; Levchenko, O.V.; Andreev, D.N.; Sokolov, F.S.; Fomenko, A.K.; Devkota, M.K.; Andreev, N.G.; Zaborovsky, A.V.; Tsaregorodtsev, S.V.; Evdokimov, V.V.; Bely, P.A.; Maev, I.V.; Govorun, V.M.; Ilina, E.N.

Авторы: Starikova Elizaveta V., Galeeva Yulia S., Fedorov Dmitry E., Korneenko Elena V., Speranskaya Anna S., Selezneva Oksana V., Zoruk Polina Y., Klimina Ksenia M., Veselovsky Vladimir A., Morozov Maxim D., Boldyreva Daria I., Olekhnovich Evgenii I., Manolov Alexander I., Pavlenko Alexander V., Kozlov Ivan E., Yanushevich Oleg O., Krikheli Natella I., Levchenko Oleg V., Andreev Dmitry N., Sokolov Filipp S., Fomenko Aleksey K., Devkota Mikhail K., Andreev Nikolai G., Zaborovsky Andrey V., Tsaregorodtsev Sergei V., Evdokimov Vladimir V., Bely Petr A., Maev Igor V., Govorun Vadim M., Ilina Elena N.
Журнал: iScience
Том: 27
Номер: 12
Год издания: 2024
Издательство: Cell Press
Местоположение издательства: United States
Номер статьи: 111319
DOI: 10.1016/j.isci.2024.111319
Аннотация: Antimicrobial resistance poses a serious threat to global public health. The COVID-19 pandemic underscored the need to monitor the dissemination of antimicrobial resistance genes and understand the mechanisms driving this process. In this study, we analyzed changes to the oropharyngeal and fecal resistomes of patients with COVID-19 undergoing therapy in a hospital setting. A targeted sequencing panel of 4,937 resistance genes was used to comprehensively characterize resistomes. Our results demonstrated that the oropharyngeal resistome is homogeneous, showing low variability over time. In contrast, fecal samples clustered into two distinct resistotypes that were only partially related to enterotypes. Approximately half of the patients changed their resistotype within a week of therapy, with the majority transitioning to a less diverse and ermB-dominated resistotype 2. Common macrolide resistance genes were identified in over 80% of both oropharyngeal and fecal samples, likely originating from streptococci. Our findings suggest that the fecal resistome is a dynamic system that can exist in certain “states” and is capable of transitioning from one state to another. To date, this is the first study to comprehensively describe the oropharyngeal resistome and its variability over time, and one of the first studies to demonstrate the temporal dynamics of the fecal resistotypes.
Добавил в систему: Сперанская Анна Сергеевна

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Oropharyngeal resistome remains stable during COVID-19 therapy, while fecal resistome shifts toward a less diverse resistotypeстатья