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Moisture and Temperature as Factors of the Formation and Functioning of Prokaryotic Complexes in Oil-Contaminated Soilsстатья Исследовательская статья
- Авторы: Manucharova N.A., Ovchinnikova E.A., Kovalenko M.A., Alexeeva M.G., Smagin A.V., Rozanova M.S., Pavlov K.V., Nazina T.N., Ershov A.P., Stepanov A.L.
- Журнал: Eurasian Soil Science
- Том: 57
- Номер: 1
- Год издания: 2024
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 57
- Последняя страница: 67
- DOI: 10.1134/S1064229324602609
- Аннотация: The structure of prokaryotic complexes in microcosms of oil-contaminated and control soil samples(chernozem, Voronezh oblast, gray forest soil, Tula oblast, and chestnut soil, Volgograd oblast) was studiedunder conditions of different matric potentials of soil water and soil temperatures using molecular biologymethods (RT-PCR, metabarcoding, FISH). An increase in the content of functional genes responsible forthe synthesis of catechol-2,3-dioxygenase (xylE) and alkane monooxygenase (alkB) marking the initial stageof hydrocarbon degradation was observed in the studied oil-contaminated soils against the background of adecrease in the biomass and diversity of bacteria relative to the control. It was found that an increase in thematric potential of soil water from –800 to 0 kPa, simultaneously with an increase in the number of metabolicallyactive representatives of bacteria and the number of alkB gene copies in the oil-contaminated soils incubatedat 28°C corresponded to a decrease in the residual content of alkanes to 51%. The maximum contentof the alkB gene was observed at the total water capacity and a medium incubation temperature (28°C). Thehighest number of copies of the xylE gene was found at medium values of the matric potential of soil waterand temperature. The most resistant to oil pollution genera of bacteria were determined for every studiedvalue of the matric potential of soil water and temperature. Representatives of Pseudomonadota becamedominant under the conditions of low temperature and high water content, and spore-forming Actinomycetotaand Bacillota became dominant at increased temperature and decreased water content. The obtainedresults on the abundance of metabolically active prokaryotic complex of soils and its taxonomic diversity andbiotechnological potential can be useful for the development of efficient strategies for bioremediation of oilcontaminatedareas.
- Добавил в систему: Манучарова Наталия Александровна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | ESS57.pdf | ESS57.pdf | 883,7 КБ | 25 апреля 2025 [N.A.Manucharova] | |
| 2. | Направление | Бланк_направл.pdf | 303,5 КБ | 12 мая 2025 [N.A.Manucharova] | |
| 3. | Экспертное заключение | Эксп_заключение.pdf | 349,4 КБ | 12 мая 2025 [N.A.Manucharova] |