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Recombinant TBEV Protein E of the Siberian Subtype Is a Candidate Antigen in the ELISA Test System for Differential Diagnosisстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Baryshnikova V., Turchenko Y., Tuchynskaya K., Belyaletdinova I., Butenko A., Dereventsova A., Ignatiev G., Kholodilov I., Larichev V., Lyapeykova E., Rogova A., Shakaryan A., Shishova A., Gmyl A., Karganova G.
- Журнал: DIAGNOSTICS
- Том: 13
- Номер: 20
- Год издания: 2023
- Номер статьи: 3277
- DOI: 10.3390/diagnostics13203277
- Аннотация: The tick-borne encephalitis virus (TBEV) is one of the most common members of theOrthoflavivirus genus, which comprises the causative agents of severe diseases in humans and animals.Due to the expanding areas of orthoflavivirus infection, its differential diagnosis is highly demanded.Commercial test kits based on inactivated TBEV may not provide reliable differentiation betweenflaviviruses because of serological crossover in this genus. Application of recombinant domains(sE and dIII) of the TBEV Sukhar-strain protein E as antigens in an ELISA test system allowed usto identify a wide range of antibodies specific to different TBEV strains. We tested 53 sera fromhuman patients with confirmed TBE diagnosis (the efficacy of our test system based on sE proteinwas 98%) and 56 sera from patients with other orthoflavivirus infections in which no positive oneswere detected using our ELISA test system, thus being indicative of its 100% specificity. We alsotested mouse and rabbit sera containing antibodies specific to 17 TBEV strains belonging to differentsubtypes; this assay exhibited high efficacy and differentiation ability in detecting antibodies againstTBEV from other orthoflaviviruses such as Omsk hemorrhagic fever, Powassan, yellow fever, dengue,West Nile, Zika, and Japanese encephalitis viruses.
- Добавил в систему: Карганова Галина Григорьевна