Аннотация:Abstract: Advances in sequencing technologies and bioinformatics have led to breakthroughs inthe study of virus biodiversity. Millipedes (Diplopoda, Myriapoda, Arthropoda) include more than12,000 extant species, yet data on virus diversity in Diplopoda are scarce. This study aimed to explorethe virome of the millipedes collected in the Dong Nai Biosphere Reserve in Vietnam. We studied14 species of millipedes and managed to assemble and annotate the complete coding genomesof 16 novel viruses, the partial coding genomes of 10 more viruses, and several fragmented viralsequences, which may indicate the presence of about 54 more viruses in the studied samples. Amongthe complete and partial genomes, 27% were putative members of the order Picornavirales. Most of thediscovered viruses were very distant from the viruses currently present in the relevant databases. Atleast eight viruses meet the criteria to be recognized as a new species by the International Committeeon Taxonomy of Viruses, and, for two of them, a higher taxonomic status (genus and even family)can be suggested.