Ultrafast Photochemical Reaction of Exiguobacterium sibiricum Rhodopsin (ESR) at Alkaline pH

Smitienko, O.A.; Feldman, T.B.; Petrovskaya, L.E.; Kryukova, E.A.; Shelaev, I.V.; Gostev, F.E.; Cherepanov, D.A.; Kolchugina, I.B.; Dolgikh, D.A.; Nadtochenko, V.A.; Kirpichnikov, M.P.; Ostrovsky, M.A.

Авторы: Smitienko O.A., Feldman T.B., Petrovskaya L.E., Kryukova E.A., Shelaev I.V., Gostev F.E., Cherepanov D.A., Kolchugina I.B., Dolgikh D.A., Nadtochenko V.A., Kirpichnikov M.P., Ostrovsky M.A.
Журнал: Russian Journal of Bioorganic Chemistry
Том: 50
Номер: 4
Год издания: 2024
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 1285
Последняя страница: 1293
DOI: 10.1134/S1068162024040058
Аннотация: Abstract—Objective: Rhodopsin from the eubacterium Exiguobacterium sibiricum (ESR) performs the light-dependent proton pumping function. The operation of ESR is based on the ultrafast photochemical reaction of isomerization of the retinal chromophore, which triggers dark processes closed in a photocycle. Many parameters of the photocycle are determined by the presence of a hydrogen bond between the primary counterion Asp85 and the chromophore. ESR in detergent micelles pumps protons most efficiently at pH > 9, when such a bond is most probable. Methods: In the present study, the photochemical reaction of ESR at pH 9.5 was investigated by femtosecond laser absorption spectroscopy. Results and Discussion: It was shown that photoisomerization of the chromophore group occurs in 0.51 ps, with the contribution from the reactive excited state being ca. 80%. A comparison with the earlier obtained data for pH 7.4 showed that, at pH 9.5, the reaction proceeds much faster and more efficiently. Conclusions: The present results confirm the important role of the chromophore group counterion in the photoactivated processes of rhodopsins.
Добавил в систему: Фельдман Татьяна Борисовна

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Ultrafast Photochemical Reaction of Exiguobacterium sibiricum Rhodopsin (ESR) at Alkaline pHстатья

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