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The Quasi-Equilibrium Assumption for Bi-Bi Ordered Bisubstrate Enzymatic Reaction. How to Discriminate the Mechanism Correctlyстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 сентября 2014 г.
- Автор: Vrzheshch P.V.
- Журнал: Biochemistry (Moscow)
- Том: 75
- Номер: 11
- Год издания: 2010
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 1374
- Последняя страница: 1382
- DOI: 10.1134/S000629791011009X
- Аннотация: Application of the quasi-equilibrium assumption for the steady-state kinetics of bisubstrate irreversible enzymatic reactions in the case of ordered binding of substrates (Bi-Bi ordered mechanism) is considered. The necessary and sufficient conditions for application of the quasi-equilibrium assumption have been found and accuracy of this assumption has been numerically evaluated. The limitations on application of the quasi-equilibrium assumption have been shown and errors of its application have been analyzed. It is shown that possible discrimination of substrate binding order using asymmetrical expressions grounded on the quasi-equilibrium assumption is inconsistent because such asymmetrical expressions arise from incorrect application of the quasi-equilibrium assumption. Moreover, it has been proved in the general case that mechanisms generating such substrate-asymmetrical expressions for the steady-state rate of enzymatic reaction do not exist. The error source when using graphical interpretation for discrimination of mechanisms of bisubstrate enzymatic reactions has been determined. The strategy to avoid such errors is pointed out.
- Добавил в систему: Вржещ Петр Владимирович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | The Quasi-Equilibrium Assumption for Bi-Bi Ordered Bisubstrate Enzymatic Reaction. How to Discriminate the Mechanism Correctly | 2010_2_bcm_1374.pdf | 264,1 КБ | 15 сентября 2014 [VrzheshchPV] |