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Application of PCR-based approaches for evaluation of cell-free DNA fragmentation in colorectal cancerстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Koval AP, Khromova AS, Blagodatskikh KA, Zhitnyuk YV, Shtykova YA, Alferov AA, Kushlinskii NE, Shcherbo DS
- Журнал: Frontiers in Molecular Biosciences
- Том: 10
- Год издания: 2023
- Издательство: Frontiers Media S.A.
- Местоположение издательства: Lausanne, Switzerland
- DOI: 10.3389/fmolb.2023.1101179
- Аннотация: Cell-free DNA (cfDNA) testing is the core of most liquid biopsy assays. In particular, cfDNA fragmentation features could facilitate non-invasive cancer detection due to their interconnection with tumor-specific epigenetic alterations. However, the final cfDNA fragmentation profile in a purified sample is the result of a complex interplay between informative biological and artificial technical factors. In this work, we use ddPCR to study cfDNA lengths in colorectal cancer patients and observe shorter and more variable cfDNA fragments in accessible chromatin loci compared to the densely packed pericentromeric region. We also report a convenient qPCR system suitable for screening cfDNA samples for artificial high molecular weight DNA contamination.
- Добавил в систему: Кубасова Ирина Юрьевна