Mechanical and signaling responses of unloaded rat soleus muscle to chronically elevated β-myosin activity

Sergeeva, K.V.; Tyganov, S.A.; Zaripova, K.A.; Bokov, R.O.; Nikitina, L.V.; Konstantinova, T.S.; Kalamkarov, G.R.; Shenkman, B.S.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 апреля 2024 г.

Авторы: Sergeeva K.V., Tyganov S.A., Zaripova K.A., Bokov R.O., Nikitina L.V., Konstantinova T.S., Kalamkarov G.R., Shenkman B.S.
Журнал: Archives of Biochemistry and Biophysics
Том: 754
Номер: 1
Год издания: 2024
Издательство: Academic Press
Местоположение издательства: United States
Первая страница: 1
Последняя страница: 14
Номер статьи: 109961
DOI: 10.1016/j.abb.2024.109961
Аннотация: It has been reported that muscle functional unloading is accompanied by an increase in motoneuronal excitability despite the elimination of afferent input. Thus, we hypothesized that pharmacological potentiation of spontaneous contractile soleus muscle activity during hindlimb unloading could activate anabolic signaling pathways and prevent the loss of muscle mass and strength. To investigate these aspects and underlying molecular mechanisms, we used β-myosin allosteric effector Omecamtiv Mekarbil (OM). We found that OM partially prevented the loss of isometric strength and intrinsic stiffness of the soleus muscle after two weeks of disuse. Notably, OM was able to attenuate the unloading-induced decrease in the rate of muscle protein synthesis (MPS). At the same time, the use of drug neither prevented the reduction in the markers of translational capacity (18S and 28S rRNA) nor activation of the ubiquitin-proteosomal system, which is evidenced by a decrease in the cross sectional area of fast and slow muscle fibers. These results suggest that chemically-induced increase in low intensity spontaneous contractions of the soleus muscle during functional unloading creates prerequisites for protein synthesis. At the same time, it should be assumed that the use of OM is advisable with pharmacological drugs that inhibit the expression of ubiquitin ligases.
Добавил в систему: Боков Роман Олегович

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Mechanical and signaling responses of unloaded rat soleus muscle to chronically elevated β-myosin activityстатья

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