Histidine-Bound Dinitrosyl Iron Complexes: Antioxidant and Antiradical Properties

Shumaev, K.B.; Kosmachevskaya, O.V.; Nasybullina, E.I.; Ruuge, E.K.; Kalenikova, E.I.; Topunov, A.F.

Авторы: Shumaev K.B., Kosmachevskaya O.V., Nasybullina E.I., Ruuge E.K., Kalenikova E.I., Topunov A.F.
Журнал: International Journal of Molecular Sciences
Том: 2023
Номер: 24
Год издания: 2023
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 17236
Последняя страница: 10
DOI: 10.3390/ijms242417236
Аннотация: Dinitrosyl iron complexes (DNICs) are important physiological derivatives of nitric oxide.These complexes have a wide range of biological activities, with antioxidant and antiradical onesbeing of particular interest and importance. We studied the interaction between DNICs associatedwith the dipeptide L-carnosine or serum albumin and prooxidants under conditions mimickingoxidative stress. The ligands of these DNICs were histidine residues of carnosine or His39 and Cys34in bovine serum albumin. Carnosine-bound DNICs reduced the level of piperazine free radicalsin the reaction system containing tert-butyl hydroperoxide (t-BOOH), bivalent iron ions, a nitroxylanion donor (Angeli’s salt), and HEPES buffer. The ability of carnosine DNICs to intercept organicfree radicals produced from t-BOOH decay could lead to this effect. In addition, carnosine DNICsreacted with the superoxide anion radical (O2•−) formed in the xanthine/xanthine oxidase enzymaticsystem. They also reduced the oxoferryl form of the heme group formed in the reaction of myoglobinwith t-BOOH. DNICs associated with serum albumin were found to be rapidly destroyed in a modelsystem containing metmyoglobin and t-BOOH. At the same time, these protein DNICs inhibited thet-BOOH-induced oxidative degradation of coenzymes Q9 and Q10 in rat myocardial homogenate.The possible mechanisms of the antioxidant and antiradical action of the DNICs studied and theirrole in the metabolism of reactive oxygen and nitrogen species are discussed.
Добавил в систему: Каленикова Елена Игоревна

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Histidine-Bound Dinitrosyl Iron Complexes: Antioxidant and Antiradical Propertiesстатья

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