|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ПсковГУ |
||
Advances and obstacles in using CRISPR/Cas9 technology for non-coding RNA gene knockout in human mesenchymal stromal cellsстатья Электронная публикация
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 ноября 2023 г.
- Авторы: Basalova Nataliya, Illarionova Maria, Skryabina Mariya, Vigovskiy Maksim, Tolstoluzhinskaya Anastasia, Primak Alexandra, Chechekhina Elizaveta, Chechekhin Vadim, Karagyaur Maxim, Efimenko Anastasia
- Журнал: Non-Coding RNA
- Том: 9
- Номер: 5
- Год издания: 2023
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Номер статьи: 49
- DOI: 10.3390/ncrna9050049
- Аннотация: Non-coding RNA (ncRNAs) genes have attracted increasing attention in recent years due to their widespread involvement in physiological and pathological processes and regulatory networks. The study of the function and molecular partners of ncRNAs opens up opportunities for the earlydiagnosis and treatment of previously incurable diseases. However, the classical “loss-of-function” approach in ncRNA function analysis is challenged due to some specific issues. Here, we have studied the potency of two CRISPR/Cas9 variants, wild-type (SpCas9wt) and nickase (SpCas9D10A) programmable nucleases, for the editing of extended DNA sequences in human mesenchymal stromal cells (MSCs). Editing the genes of fibrosis-related hsa-miR-21-5p and hsa-miR-29c-3p, we have shown that a pair of SpCas9D10A molecules can effectively disrupt miRNA genes within the genomes of MSCs. This leads not only to a decrease in the level of knockout miRNA in MSCs and MSC-produced extracellular vesicles, but also to a change in cell physiology and the antifibrotic properties of the cell secretome. These changes correlate well with previously published data for the knockdown of certain miRNAs. The proposed approach can be used to knock out ncRNA genes within the genomes of MSCs or similar cell types in order to study their function in biological processes.
- Добавил в систему: Карагяур Максим Николаевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|