Structure–Activity Relationships and Transcriptomic Analysis of Hypoxia-Inducible Factor Prolyl Hydroxylase Inhibitors

Poloznikov, A.A.; Nikulin, S.V.; Hushpulian, D.M.; Khristichenko, A.Y.; Osipyants, A.I.; Asachenko, A.F.; Shurupova, O.V.; Savin, S.S.; Lee, S.H.; Gaisina, I.N.; Thatcher Gregory, R.J.; Narciso, A.; Chang, E.P.; Kazakov, S.V.; Krucher, N.; Tishkov, V.I.; Thomas, B.; Gazaryan, I.G.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 8 апреля 2022 г.

Авторы: Poloznikov Andrey A., Nikulin Sergey V., Hushpulian Dmitry M., Khristichenko Anna Yu, Osipyants Andrey I., Asachenko Andrey F., Shurupova Olga V., Savin Svyatoslav S., Lee Sue H., Gaisina Irina N., Thatcher Gregory R.J., Narciso Anthony, Chang Eric P., Kazakov Sergey V., Krucher Nancy, Tishkov Vladimir I., Thomas Bobby, Gazaryan Irina G.
Журнал: ANTIOXIDANTS
Том: 11
Номер: 2
Год издания: 2022
Номер статьи: 220
DOI: 10.3390/antiox11020220
Аннотация: To evaluate the differences in action of commercially available 2-oxoglutarate mimetics and “branched-tail” oxyquinoline inhibitors of hypoxia-inducible factor prolyl hydroxylase (HIF PHD), the inhibitors’ IC50 values in the activation of HIF1 ODD-luciferase reporter were selected for comparative transcriptomics. Structure–activity relationship and computer modeling for the oxyquinoline series of inhibitors led to the identification of novel inhibitors, which were an order of magnitude more active in the reporter assay than roxadustat and vadadustat. Unexpectedly, 2-methyl-substitution in the oxyquinoline core of the best HIF PHD inhibitor was found to be ac-tive in the reporter assay and almost equally effective in the pretreatment paradigm of the oxy-gen-glucose deprivation in vitro model. Comparative transcriptomic analysis of the signaling pathways induced by HIF PHD inhibitors showed high potency of the two novel oxyquinoline in-hibitors (#4896-3249 and #5704-0720) at 2 μM concentrations matching the effect of 30 μM roxadustat and 500 μM dimethyl oxalyl glycine in inducing HIF1 and HIF2-linked pathways. The two oxyquinoline inhibitors exerted the same activation of HIF-triggered glycolytic pathways but opposite effects on signaling pathways linked to alternative substrates of HIF PHD 1 and 3, such as p53, NF-κB, and ATF4. This finding can be interpreted as the specificity of the 2-methyl-substitute variant for HIF PHD2.
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Structure–Activity Relationships and Transcriptomic Analysis of Hypoxia-Inducible Factor Prolyl Hydroxylase Inhibitorsстатья

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