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Simultaneous express immunoassay of multiple cardiac biomarkers with anautomatic platform in human plasma0статья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 мая 2021 г.
- Авторы: Nechaeva N.L., Sorokina O.N., Konstantinova T.S., Vasilyeva A.D., Yurina L.V., Byzova N.A., Bugrova A.E., Yanovich S.V., Eremenko A.V., Kurochkin I.N.
- Журнал: Talanta
- Том: 224
- Год издания: 2021
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Номер статьи: 121860
- DOI: 10.1016/j.talanta.2020.121860
- Аннотация: C-reactive protein, cystatin C, myoglobin, and D-dimer represent the inflammatory or thromboembolic status ofthe patient and play important roles in early diagnostics of acute myocardial infarction. Each protein can indicatesome health problems, but their simultaneous detection can be crucial for differential diagnostics. The expressanalysis of these proteins in a small drop of plasma was developed using magnetic beads. The suggested methodis based on immunomagnetic extraction of the target analyte from plasma samples and its simultaneous labellingby fluorescent dye. Reaction time was optimized for quantification of cardiac biomarkers in the spike solutionsand human plasma samples. In this paper, we developed a one-protein detection technique for each cardiacbiomarker and united it to a four-protein facility using an automatic platform. The proposed technique requiresonly 17 μL of the human plasma and takes 14 min for four-protein measuring. The suggested technique coversconcentration difference by more than two orders of magnitude and demonstrates analytical applicability bymeasurements of human plasma samples of 16 volunteers.
- Добавил в систему: Курочкин Илья Николаевич
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