Hematopoietically-expressed homeobox protein HHEX regulates adipogenesis in preadipocytes

Evseeva, M.N.; Dyikanov, D.T.; Karagyaur, M.N.; Prikazchikova, T.A.; Sheptulina, A.F.; Balashova, M.S.; Zatsepin, T.S.; Rubtsov, Y.P.; Kulebyakin, K.Y.

Авторы: Evseeva Maria N., Dyikanov Daniyar T., Karagyaur Maxim N., Prikazchikova Tatyana A., Sheptulina Anna F., Balashova Maria S., Zatsepin Timofei S., Rubtsov Yury P., Kulebyakin Konstantin Y.
Журнал: Biochimie
Том: 185
Год издания: 2021
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 68
Последняя страница: 77
DOI: 10.1016/j.biochi.2021.02.011
Аннотация: Obesity is a key health problem and is associated with a high risk of type 2 diabetes and other metabolicdiseases. Increased weight as well as dysregulation of adipocyte homeostasis are the main drivers ofobesity. Pathological adipogenesis plays a central role in obesity-related complications such as type 2diabetes, hypertension and others. Thus, an understanding of the molecular mechanisms involved inphysiological and pathogenic adipogenesis can help to develop new strategies to prevent or cure obesityand related diseases. Previously, genetic polymorphisms in the HHEX gene that encodes the homeoboxtranscription factor HEX (PRH) were found to be associated with type 2 diabetes and high body mass indexat birth by GWAS in distinct human populations. To understand whether HHEX has a regulatory function inadipogenesis, we performed RNAi-mediated knockdown of Hhex in preadipocyte cell line 3T3-L1 in vitro,and studied changes in the efficacy of adipogenesis.We found that Hhex knockdown blocks adipogenesis inpreadipocytes in a dose-dependent manner and leads to a significant decrease of PPAR-gamma protein ethe main regulator of adipogenesis. We also propose that Hhex can play an important role in adipocytedifferentiation by affecting the level of the PPAR-gamma protein. Our study supports the claim that Hhexplays an important role in adipocyte differentiation program and can contribute to fat tissue homeostasis.
Добавил в систему: Евсеева Мария Николаевна

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Hematopoietically-expressed homeobox protein HHEX regulates adipogenesis in preadipocytesстатья

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