A copper nanoparticle-based electrochemical immunosensor for carbaryl detection

Dorozhko, E.V.; Gashevskay, A.S.; Korotkova, E.I.; Barek, J.; Vyskocil, V.; Eremin, S.A.; Galunin, E.V.; Saqib, M.

Авторы: Dorozhko Elena V., Gashevskay Anna S., Korotkova Elena I., Barek Jiri, Vyskocil Vlastimil, Eremin Sergei A., Galunin Evgeny V., Saqib Muhammad
Журнал: Talanta
Том: 228
Год издания: 2021
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Номер статьи: 122174
DOI: 10.1016/j.talanta.2021.122174
Аннотация: A hapten-protein conjugate with copper nanoparticles (Hap-Car-BSA@CuNPs) was first synthesized in the present work for the determination of carbaryl. The copper nanoparticles (CuNPs) of the conjugate were used as electrochemical labels in the direct solid-phase competitive analysis of carbaryl residues in flour from different crops. The signal was read by linear sweep anodic stripping voltammetry (LSASV) of copper (through the electrochemical stripping of accumulated elemental copper) on a gold-graphite electrode (GGE). To form a recognition receptor layer of monoclonal antibodies against the carbaryl on the surface of GGE, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 1-hydroxy-2,5-pyrrolidinedione (NHS) were used as the best covalent cross-linkers. The concentrations of the antibodies and the Hap-Car-BSA@CuNPs conjugate were optimized for carbaryl detection by the electrochemical immunosensor. The electrochemical immunosensor can be used for highly sensitive determination of carbaryl residues in flour samples in the concentration range 0.8-32.3 μg∙kg-1 with a limit of detection 0.08 μg kg-1. The present work paves the path for a novel method for monitoring carbaryl in other food products, drinks and soil samples.A hapten-protein conjugate with copper nanoparticles (Hap-Car-BSA@CuNPs) was first synthesized in the present work for the determination of carbaryl. The copper nanoparticles (CuNPs) of the conjugate were used as electrochemical labels in the direct solid-phase competitive analysis of carbaryl residues in flour from different crops. The signal was read by linear sweep anodic stripping voltammetry (LSASV) of copper (through the electrochemical stripping of accumulated elemental copper) on a gold-graphite electrode (GGE). To form a recognition receptor layer of monoclonal antibodies against the carbaryl on the surface of GGE, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 1-hydroxy-2,5-pyrrolidinedione (NHS) were used as the best covalent cross-linkers. The concentrations of the antibodies and the Hap-Car-BSA@CuNPs conjugate were optimized for carbaryl detection by the electrochemical immunosensor. The electrochemical immunosensor can be used for highly sensitive determination of carbaryl residues in flour samples in the concentration range 0.8-32.3 μg∙kg-1 with a limit of detection 0.08 μg kg-1. The present work paves the path for a novel method for monitoring carbaryl in other food products, drinks and soil samples.
Добавил в систему: Еремин Сергей Александрович

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A copper nanoparticle-based electrochemical immunosensor for carbaryl detectionстатья

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