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mRNA containing an extended Shine-Dalgarno sequence is translated independently of ribosomal protein S1статья
Информация о цитировании статьи получена из
Scopus
Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
- Авторы: Balakin AG, Bogdanova SL, Skripkin EA
- Журнал: Biochemstry International
- Том: 27
- Номер: 1
- Год издания: 1992
- Первая страница: 117
- Последняя страница: 129
- DOI: ISSN: 0158-5231
- Аннотация: The role of ribosomal protein S1 in the translation of mRNA containing an extended Shine-Dalgarno sequence was investigated. Using the toeprinting technique, formation of the ternary initiation complex between 30S subunits, both S1-depleted or treated with anti-S1 antibodies, and mini-mRNA containing the 9 nucleotide-long Shine-Dalgarno sequence was studied. It was concluded that the initiation of translation on mRNA with an extended Shine-Dalgarno sequence is S1-independent. It was demonstrated that S1-depleted ribosomes effectively translate the cro-mini-mRNA in a cell-free system. In contrast to cro-mini-mRNA, 30S subunits without protein S1 are inactive in ternary initiation complex formation with, and cell-free translation of, MS2 or fr phage RNAs and RNA protein III of phage fd.
- Добавил в систему: Скрипкин Евгений Алексеевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | 1992_Biochemistry_International_27-1-117.PDF | 9,2 МБ | 8 ноября 2015 [SkripkinEA] |