Prostaglandins E2 and D2 – regulators of host immunity in model parasite Diphyllobothrium dendriticum: immunocytochemical and biochemical study

Kutyrev, I.A.; Biserova, N.M.; Olennikov, D.N.; Korneva, J.V.; Mazur, O.E.

Авторы: Kutyrev Ivan A., Biserova Natalia M., Olennikov Daniil N., Korneva Janetta V., Mazur O.E.
Журнал: Molecular and Biochemical Parasitology
Том: 212
Год издания: 2017
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 33
Последняя страница: 45
DOI: 10.1016/j.molbiopara.2017.01.006
Аннотация: Abstract The spectrum of immunomodulating molecules produced by tapeworms is to date not well understood. The aims of this study on the tapeworm Diphyllobothrium dendriticum were 1) detection and quantification of prostaglandins (PGs) E2 and D2 by high performance liquid chromatography; 2) appearance of PGE2 and PGD2 in particular cells by methods of immunocytochemistry and confocal laser scanning microscopy, 3) investigation of the ultrastructure of the potential cells producing PGE2 and PGD2. PGE2 immunoreaction (IR) has been found in the apical terminals of the frontal glands and sensory organs in the tegument; and in small neurons belonging to the main cords and exiting commissures. PGE2-IR partly coincided with α-tubulin-IR. PGD2-IR occurred in the muscle fibers of longitudinal and transversal body muscles and coincided with phalloidin TRITC staining. Both PGE2 and PGD2 were revealed in the flame cells of the excretory system. The ultrastructural study of the tegument revealed two types of structures which potentially produced PGE2: ciliated and unciliated free nerve endings and the frontal gland terminals reinforced with neurotubules. In the main nerve cords, large and small interneurons have been found, and the small neurons were identified as potentially exhibiting PGE2-immunoreactivity . In homogenates of the plerocercoids the evaluated content of PGE2 and PGD2 amounted to 33.15 ng•mg-1 and 1.94 ng•mg-1 of fresh tissue weight. Our previous studies demonstrated that in vitro similar PGE2 concentrations induced a strong immunosuppressive effect on fish leucocytes viability and reactive oxygen species production (Kutyrev et al., 2014). Thus, prostaglandins revealed in D. dendriticum most probably play a dual role: i) PGE2 and PGD2 potentially modulated the Coregonus autumnalis antiparasitic immune response switching it from Th1 to Th2 responses; ii) PGE2 presumably is necessary for proper development and function of the nervous system, and PGD2 can act as antagonist against mediators causing muscle contraction. Keywords: Cestoda, prostaglandins, immunomodulators, glands, neurons, sensilla, flame cells, muscles.
Добавил в систему: Бисерова Наталья Михайловна

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Prostaglandins E2 and D2 – regulators of host immunity in model parasite Diphyllobothrium dendriticum: immunocytochemical and biochemical studyстатья

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