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Kinetic mechanism of the bifunctional enzyme prostaglandin-H-synthase. Effect of electron donors on the cyclooxygenase reactionстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 7 октября 2013 г.
- Авторы: Tsaplina L.A., Karatasso Y.O., Filimonov I.S., Vrzheshch P.V.
- Журнал: Biochemistry (Moscow)
- Том: 71
- Номер: 11
- Год издания: 2006
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 1247
- Последняя страница: 1255
- DOI: 10.1134/S0006297906110101
- Аннотация: ProstaglandinHsynthase (PGHS, EC 1.14.99.1) catalyzes the first committed step in biosynthesis of all prostaglandins, thromboxanes, and prostacyclins by converting arachidonic acid to prostaglandin H2 (PGH2). PGHS exhibits two enzymatic activities: cyclooxygenase activity converting arachidonic acid to prostaglandin G2 (PGG2) and peroxidase activity reducing the hydroperoxide PGG2 to the corresponding alcohol, PGH2. Despite the many investigations of the kinetics of PGHS, many features such as the absence of competition and mutual activation between the cyclooxygenase and peroxidase activities cannot be explained in terms of existing schemes. In this work we have studied the influence of different electron donors (N,N,N′,N′tetramethylpphenylenediamine, Lepinephrine, 2,2′azinobis(3-ethylbenzthiazoline6sulfonic acid), potassium ferrocyanide) on the PGHS activities. The proposed scheme describes independent but interconnected cyclooxygenase and peroxidase activities of PGHS. It also explains the experimental data obtained in the present work and known from the literature.
- Добавил в систему: Филимонов Иван Сергеевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Kinetic mechanism of the bifunctional enzyme prostaglandin-H-synthase. Effect of electron donors on the cyclooxygenase reaction | 2006_bcm_1247_1255_Tsaplina_Karatasso_Filimonov_Vrzheshch.p… | 200,8 КБ | 15 сентября 2014 [VrzheshchPV] |