Proteomic analysis of Trichinella spiralis and Trichinella pseudospiralis muscle larval excretory-secretory proteinsстатьяТезисы
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Дата последнего поиска статьи во внешних источниках: 10 июня 2020 г.
Аннотация:Trichinella are tissue parasitic nematodes that cause zoonotic disease of mammals, reptiles and birds. The main role in the development of the pathological process in trichinellosis belongs to protein compounds secreted by these helminths, the study of the qualitative and quantitative composition of which is of priority for medicine and veterinary science. Larvae of T. spiralis strain MH259593 (originally isolated from the muscles of domestic pig) and T. pseudospiralis strain KU357408 (isolated from raccoon muscles) were maintained by repeated passaging on white mongrel rats, from the muscles of which the biomass of larvae was obtained for further cultivation in DMEM. Excretory-secretory (E-S) products were collected after 48 h of cultivation and analysed by liquid chromatography tandem mass spectrometry (LS-MS/MS). Bioinformatics analysis was also applied to the obtained results. Multiple functional proteins were revealed in E-S products of both strains: more than 150 proteins in T. spiralis and more than 270 proteins in T. pseudospiralis, among them proteins previously revealed by us earlier (Odoevskaya et al., 2018). These proteins belonged to deoxyribonuclease family proteins, 5’-nucleotidases and serine proteases, which showed different degrees of identity with similar proteins previously found in E-S products of Trichinella by other authors. Our results show that the protein spectrum in E-S products can vary both between Trichinella spp., as well as between strains. The probable sources of such variability at the genome level of Trichinella are discussed