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Prostaglandin H synthase kinetics in the two-phase aqueous-micellar systemстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 января 2019 г.
- Авторы: Ponomareva Olga A., Trushkin Nikita A., Filimonov Ivan S., Krivoshey Alexandr V., Barkhatov Vladimir I., Mitrofanov Sergey I., Vrzheshch Petr V.
- Журнал: Biochimica et Biophysica Acta - Biomembranes
- Том: 1858
- Номер: 9
- Год издания: 2016
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 2199
- Последняя страница: 2207
- DOI: 10.1016/j.bbamem.2016.06.013
- Аннотация: Reaction mixture for PGHS (prostaglandin-H-synthase) is a two-phase system including micellar hydrophobic phase and hydrophilic aqueous phase. Reagents added to the mixture are distributed between phases, thus concentrations of reagents dissolved in phases can differ significantly from their overall contents. Using dynamic light scattering we found that the hydrophobic phase produced by tween-20 consists of micelles, which radius (4–5 nm) does not depend on either tween-20 overall content (0.1%–1%v/v) or arachidonic acid (AA) addition (10–1000 μM) or PGHS addition (1 μM). Tween-20 overall content changing from 0.1% to 2%v/v dramatically affected COX kinetic, but accounting AA distribution between phases allowed us to estimate “true” parameters, independent of the tween-20 overall content and the concentration of another substrate: KMOx equals 9.8 μM O2 in the aqueous phase or 0.0074 bar in the gaseous phase, KMAA equals 5400 μM AA in the phase of tween-20 micelles and 5400/P μM AA in the aqueous phase (P is the distribution ratio for the AA between the aqueous phase and the hydrophobic phase (P > > 1000)). This approach allowed to evaluate PS, the distribution ratio for the AA between the hydrophobic phase and the PGHS active center (PS ~ 310). This coefficient indicates the AA selectivity toward the cyclooxygenase active center.
- Добавил в систему: Митрофанов Сергей Игоревич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Manuscript | 1-s2.0-S0005273616302218-main.pdf | 641,8 КБ | 28 июня 2016 [Mitrofanov] |