DEVELOPMENT OF FLUORESCENCE POLARISATION AND ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR DANOFLOXACIN DETECTION IN MILK

Авторы: SHANIN I.A., ZVEREVA E.A., ZHERDEV A.V., EREMIN S.A., DZANTIEV B.B.
Журнал: Int. J. Chem. Sci
Том: 14
Номер: 12
Год издания: 2016
Первая страница: 283
Последняя страница: 298
Аннотация: Two selective immunotechniques based on heterologous approach were proposed and chacterized to detect danofloxacin, a priority veterinary contaminant, in milk. The first technique is fluorescence polarisation immunoassay (PFIA), where a danofloxacin tracer was synthesised with 4-aminomethyl fluorescein, with polyclonal antibodies obtained for a danofloxacin conjugate with cationised bovine serum albumin. The developed system has a detection limit for danofloxacin equal to 13 ng/mL and does not show any cross-reactivity with other 24 tested fluoroquinolones. The second assay format is enzymelinked immunosorbent assay (ELISA) for danofloxacin that is based on the application of the same antibodies and a heterologous solid-phase clinafloxacin conjugate with ovalbumine. The limit of danofloxacin detection for the given ELISA is 0.5 ng/mL, and its strong selectivity to danofloxacin has been also confirmed. The possibility of quantitative control of danofloxacin content in milk has been shown for the developed ELISA technique; the opening percentage was in the range from 83 to 130%.
Добавил в систему: Еремин Сергей Александрович

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