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Chiral high-performance liquid chromatography analysis of α-amino acid mixtures using a novel SH reagent-N-R-mandelyl-l-cysteine and traditional enantiomeric thiols for precolumn derivatizationстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Chernobrovkin M.G., Shapovalova E.N., Guranda D.T., Kudryavtsev P.A., Švedas V.K., Shpigun O.A.
- Журнал: Journal of Chromatography A
- Том: 1175
- Номер: 1
- Год издания: 2007
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 89
- Последняя страница: 95
- DOI: 10.1016/j.chroma.2007.10.034
- Аннотация: Several chiral thiols, i.e. traditionally used enantiomerically pure SH reagents and novel N-R-mandelyl-l-cysteine (R-NMC) containing additional chiral center, have been applied as co-reagents in precolumn derivatization with o-phthalaldehyde for enantiomeric HPLC analysis of individual α-amino acids and their mixtures. The R-NMC-derived isoindoles as well as adducts with other thiols have a characteristic absorption maximum at 340 nm, and are highly fluorescent allowing detection of 10 μg/l of an amino acid. Investigated 19 amino acids were analyzed separately and in a mixture by a gradient HPLC after precolumn derivatization. The chromatographic behavior of formed isoindoles substantially differs for each of the thiols used for modification. In contrast to traditional enantiomeric thiols application of diastereomeric R-NMC provides higher resolution for α-amino acid enantiomers, with l,d-elution order (except for Arg). Combined use of R-NMC and other thiol enlarges the possibilities of this method, allowing accurate chiral analysis of complex amino acid mixtures.
- Добавил в систему: Шаповалова Елена Николаевна