|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ПсковГУ |
||
Electrosynthesis and binding properties of molecularly imprinted poly-o-phenylenediamine for selective recognition and direct electrochemical detection of myoglobinстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 декабря 2016 г.
- Авторы: Shumyantseva V.V., Bulko T.V., Sigolaeva L.V., Kuzikov A.V., Archakov A.I.
- Журнал: Biosensors and Bioelectronics
- Том: 86
- Год издания: 2016
- Издательство: Pergamon Press Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: 330
- Последняя страница: 336
- DOI: 10.1016/j.bios.2016.05.101
- Аннотация: Electrosynthesis of molecularly imprinted polymer (MIP) templated with myoglobin (Mb) and the reference non-imprinted polymer (NIP) was examined with o-phenylenediamine (o-PD) as a monomer. Mass-sensitive quartz crystal microbalance with dissipation monitoring supplied by an electrochemical module (EQCM-D) was applied to characterize and optimize MIP/NIP electrosynthesis. Mb rebinding was detected by direct electrocatalytic reduction of Mb by square wave voltammetry (SWV) or differential pulse voltammetry (DPV). The results obtained showed high specificity of polymeric antibodies to template Mb, with an imprinting factor determined as a ratio Imax(MIP)/Imax(NIP) of 2–4. The prepared MIP sensor is characterized by an apparent dissociation constant of (3.3±0.5)×1E−09 M and has a broad range of working concentrations of 1 nM–1 μМ, with the detection limit of 0.5 nM (9 ng/ml). Mb rebinding was examined in Mb-free diluted human serum spiked with Mb as well as in plasma samples of patients with acute myocardial infarction (AMI) and in control plasma of healthy donors in order to demonstrate the potential medical application of developed MIP sensors.
- Добавил в систему: Сиголаева Лариса Викторовна