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RNA isolation with low toxic ammonium trichloroacetateстатья
- Авторы: Kondakova Olga, Gavryushina Elena, Hallan Vippin, Drygin Yuri
- Журнал: European Journal of Biotechnology and Bioscience
- Том: 4
- Номер: 4
- Год издания: 2016
- Первая страница: 11
- Последняя страница: 19
- Аннотация: A method of RNA extraction with ammonium trichloroacetate (ATCA), the low toxic chaotropic agent, and the filter paper (cotton wool) for the RNA purification from ATCA-propanol-2 solution was developed. The yield and purity of total RNA isolated from plant and animal viruses, E.coli, from animal cells and plants were identical to those obtained by the conventional methods using toxic and hazardous agents such as guanidine thiocyanate, phenol, chloroform, etc. The quality of RNA was evaluated by the comparison of their optical and electrophoretic characteristics with those obtained after SDS-phenol deproteinization. Mass molecular diagnostics of virus and viroid infections was performed using home-made hand juice squeezer with changeable plastic shells and a vacuum manifold with mini column nests compatible with the immune plate. Use of low toxic ATCA is of particular importance for quality RNA isolation from all kinds of tissues and mass molecular diagnosis of the infecting pathogens (epiphytotic, epizootics, pandemics).
- Добавил в систему: Дрыгин Юрий Федорович