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Slow Ca2+-induced inactive/active transition of the energy-dependent Ca2+ transporting system of rat liver mitochondria: clue for Ca2+ influx cooperativityстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Kasparinsky F.O., Vinogradov A.D.
- Журнал: FEBS Letters
- Том: 389
- Год издания: 1996
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 293
- Последняя страница: 296
- Аннотация: Rat liver mitochondria essentially free of endogenous Ca2+ show low initial rate of energy-dependent Ca2+ uptake. Preincubation of mitochondria under de-energized conditions in the presence of small amounts of external Ca2+ results in a 8-10-fold time-dependent increase of energy-dependent Ca2+ uptake. Ca2+-dependent activation of the Ca2+-transporting system follows first-order kinetics (t1/2 1 min in the presence of 5 ?M Ca2+ at 20°C). Ca2+-activated mitochondria demonstrate a simple hyperbolic initial rate-Ca2+ concentration dependence, whereas strong apparent cooperativity is observed in the velocity-substrate curves for Ca2+-depleted mitochondria. It is concluded that apparent cooperativity of the energy-dependent Ca2+ uptake is due to slow (as compared with the 'turnover number') activation of a Ca2+-specific uniporter which is inactive in the absence of external Ca2+.
- Добавил в систему: Каспаринский Феликс Освальдович