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Competitive ELISA of chloramphenicol: Influence of immunoreagent structure and application of the method for the inspection of food of animal originстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Kolosova A.Yu, Samsonova J.V., Egorov A.M.
- Журнал: Food and Agricultural Immunology
- Том: 12
- Номер: 2
- Год издания: 2000
- Издательство: Taylor & Francis
- Местоположение издательства: United Kingdom
- Первая страница: 115
- Последняя страница: 125
- DOI: 10.1080/095401000404067
- Аннотация: An indirect competitive ELISA for the detection of chloramphenicol (CAP) in food of animal origin (milk, meat, eggs) is described. Influence of immunoreagent structure and composition on the assay sensitivity and specificity was investigated. Two CAP derivatives were used for conjugation with proteins: CAP succinate and a diazo derivative of CAP. Molar incorporation of CAP into the coating conjugates was also varied. To eliminate matrix effect on the assay results, a special casein-containing buffer was used for milk samples, whereas for meat and egg samples a 50-fold dilution of the buffer extracts was needed. The method developed permits CAP concentrations to be determined in the range 0.08-100 μg l-1. The detection limit is 0.08 μg kg-1. Recovery in different food samples averages between 70 and 130%. The method can be applied for inspection of food of animal origin for CAP residues.
- Добавил в систему: Самсонова Жанна Васильевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | CAP_FAI_12_115.pdf | 155,5 КБ | 10 июня 2014 [jvs] |