ИСТИНА

Cell shape changes and collective cell migrations are key mechanisms of the morphogenesis of tissues and organs. During animal development, these cell behaviors are highly coordinated and regulated. The central mechanism of these coordinations and regulations is interaction between cell adhesion and mechanical stress. It was shown that main sensors during mechanically activated cell migrations or extracellular matrix synthesis are mechanically gated ion channels. But practically all these data were obtain on in vitro models, and there are not evidences that these mechanisms could drive animal development in vivo. The aim of our work was to explore expression of mechanically gated ion channels during Xenopus tropicalis embryogenesis and identify, which of them could be involved in mechanically regulated morphogenesis. So far, we have checked the following ion channels: TRPV1, TRPV2, TRPV4, TRPM3, TRPM7, TRPC1-like, TRPC5, TRPC6, PKD2, Piezo1 and Piezo2. Maternally expressed following: TRPV1, TRPV4, TRPC6, TRPM7, Piezo1 and Piezo2. Relatively high expression level was detected for TRPV4, TRPM7 and Piezo1. TRPV2, TRPM3, TRPC1-like and PKD2 start express late: from neurula or hatching stage.We have shown that early morphogenesis could be regulated by mechanical stresses through mechanically gated ion channels and in future it should proved by gain and loss of function experiments.