ИСТИНА

There are three species from Galium glaucum group in an area of our study: G. xeroticum Soo, G. bieberstenii Ehrend., G. octonarium Pobed. Only G.xeroticum have a good morphology character. We investigate ISSR markers variability in 20 natural populations. A high variability was observed in all populations (65% of variance in populations, 35% among them). We have found unique bands for regions and for one of species. A genetic discontinuity was found around G. xeroticum populations and in chalky soil areas. There are two main clusters in STRUCTURE version 2.3.4. The first is G. xeroticum cluster. The second is other populations cluster. There is a common genetic pool for G. octonarium and G. bieberstenii in Eastern Europe. Moreover, we observe a G. xeroticum genetic impact in nearest populations of other species. This influence corresponds with geographic distance.