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In the present work we study the ensembles of nanoparticles fabricated through picosecond laser ablation in distilled water in the course of irradiation of monocrystalline and porous silicon, and silicon micropowders. The size of such particles varies from 2 to 300 nm depending on the type of target used for ablation. Analysis of the produced nanoparticles Raman spectra revealed high level of their crystallinity: more than 88% for all cases. Spectrophotometry measurements of the fabricated silicon nanoparticles revealed relatively high scattering coefficient in the spectral range 400 – 1000 nm. Optical coherence tomography imaging of the suspensions drops administered on agar gel surfaces indicated high efficiency of the nanoparticles as contrast agents providing contrast up to 30 dB. The reported results demonstrated the perspectives of fabricated nanoparticles suspensions as agents for controlling optical properties in photonics and biomedical applications.