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There are large field of problems that regenerative medicine is have to solve, which needs more new techniques to fabricate scaffolds for tissue engineering. One of these new methods is electrospinning which allows defining scaffold structure and properties. Also it is crucial to select a biocompatible material with the complex of appropriate physical and chemical characteristics. Bombyx mori silk fibroin suits all of these parameters. Thus, the main goal of this investigation was to fabricate silk fibroin scaffolds by casting method and electrospinning method and comparative analysis of scaffold properties in vitro and in vivo.