ИСТИНА

Kinesin and dynein microtubule-associated proteins have been showed to play an important role in the intracellular transport. Here we examined the role of kinesin Eg5 and dynein proteins in the mechanisms of short-term depression of acetylcholine-induced chloride current (ACh-current) in command neurons of Helix lucorum. Repeated applications of acetylcholine to a cell body of command neuron imitated the protocol of tactile stimulation evoking behavioral habituation of the withdrawal reaction mediated by these neurons. We found that a dynein inhibitor decreased the ACh-current depression rate, kinesin Eg5 III and Eg5 inhibitor V reduced the initial rate of depression. The results of electrophysiological experiments in combination with mathematical modelling provided evidence that dynein and kinesin Eg5 proteins are involved in the recycling of Ach-receptors in command neurons of H. lucorum on the cellular analogue of habituation. Furthermore, these results suggest that the reciprocal interaction between dynein and kinesin Eg5 proteins located on the same vesicle can lead to reversal of their usual direction of transport (dyneins - in exocytosis and kinesin Eg5 - in endocytosis).